human colon cancer epithelial cell line (caco-2 Search Results


human colonic epithelial adenocarcinoma cell line caco2 subclone tc7  (Robert Bosch)
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Robert Bosch human colonic epithelial adenocarcinoma cell line caco2 subclone tc7
HNP-4 1 – 11 and HNP-4 1 – 11 mod show only minor cytotoxic and hemolytic activity at high concentrations. We investigated the cytotoxic activity of HNP-4 1 – 11 and HNP-4 1 – 11 mod against (A) <t>CaCo2/TC7</t> or (B) HT29 MTX E 29 cells. We seeded 1500 cells per well and treated them after 24 h with different peptide concentrations. Living cells were determined after 96 h treatment using a CellTiter Glo2.0 assay. (C) Hemolytic activity on human erythrocytes of the peptides compared to 0.1% Triton-X treatment. (A,B) Results from three independent experiments with ±SEM are shown, and (C) Results from two independent experiments with ±SEM are represented.
Human Colonic Epithelial Adenocarcinoma Cell Line Caco2 Subclone Tc7, supplied by Robert Bosch, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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HNP-4 1 – 11 and HNP-4 1 – 11 mod show only minor cytotoxic and hemolytic activity at high concentrations. We investigated the cytotoxic activity of HNP-4 1 – 11 and HNP-4 1 – 11 mod against (A) CaCo2/TC7 or (B) HT29 MTX E 29 cells. We seeded 1500 cells per well and treated them after 24 h with different peptide concentrations. Living cells were determined after 96 h treatment using a CellTiter Glo2.0 assay. (C) Hemolytic activity on human erythrocytes of the peptides compared to 0.1% Triton-X treatment. (A,B) Results from three independent experiments with ±SEM are shown, and (C) Results from two independent experiments with ±SEM are represented.

Journal: Frontiers in Microbiology

Article Title: Fragmentation of Human Neutrophil α-Defensin 4 to Combat Multidrug Resistant Bacteria

doi: 10.3389/fmicb.2020.01147

Figure Lengend Snippet: HNP-4 1 – 11 and HNP-4 1 – 11 mod show only minor cytotoxic and hemolytic activity at high concentrations. We investigated the cytotoxic activity of HNP-4 1 – 11 and HNP-4 1 – 11 mod against (A) CaCo2/TC7 or (B) HT29 MTX E 29 cells. We seeded 1500 cells per well and treated them after 24 h with different peptide concentrations. Living cells were determined after 96 h treatment using a CellTiter Glo2.0 assay. (C) Hemolytic activity on human erythrocytes of the peptides compared to 0.1% Triton-X treatment. (A,B) Results from three independent experiments with ±SEM are shown, and (C) Results from two independent experiments with ±SEM are represented.

Article Snippet: Experiments were conducted with the human colonic epithelial adenocarcinoma cell line CaCo2 subclone TC7 which was obtained from the Robert-Bosch-Hospital Stuttgart, Germany.

Techniques: Activity Assay